Expression data from mouse heart deficient of HDAC3

Gene expression changes in the heart of MCH3-KO mouse (HDAC3 f/f, Muscle Creatine Kinase-Cre) versus control WT mouse (HDAC3 f/f). Histone deacetylases (HDACs) play important roles in cardiac development and function. We show here that mice deficient of HDAC3 in heart and skeletabl muscle are relatively normal on normal chow, but develop hypertrophic cardiomyopathy and heart failure that leads to death on high-fat diet. This microarray experiment is to explore the underlying molecular mechanism. Hearts from 6-weeks old WT and MCH3-KO C57BL/6 mice (n=4 in each group, male) on normal chow were subjected to RNA extraction and Affymetrix Mouse Gene 1.0ST analysis.MCH3-KO小鼠（HDAC3 f/f，肌肉型肌酸激酶-Cre）心脏与野生型对照小鼠（HDAC3 f/f）的基因表达差异分析。组蛋白去乙酰化酶（Histone deacetylases, HDACs）在心脏发育与心脏功能中发挥重要调控作用。本研究显示，心脏与骨骼肌中HDAC3缺陷的小鼠在正常饲料喂养下表型相对正常，但高脂饮食喂养时会诱发肥厚型心肌病与心力衰竭，最终导致死亡。本次基因芯片实验旨在探索该现象背后的潜在分子机制。实验采集了正常饲料喂养的6周龄雄性C57BL/6背景野生型与MCH3-KO小鼠（每组n=4）的心脏组织，提取总RNA后进行Affymetrix小鼠基因1.0ST芯片分析。