Bile acid-dependent transcription factors and chromatin accessibility determine regional heterogeneity of intestinal antimicrobial peptides

The newly generated scRNA-seq data and bulk RNA-seq data are provided here: 1. ScRNA-seq data. To investigate the regional heterogeneity of human intestine, 14 bioptic samples from human duodenum, jejunum and ileum were collected from 10 healthy donors recruited at the time of routine gastroscopy or enteroscopy or colonoscopy for single cell RNA sequencing. Healthy volunteers were individuals without gastrointestinal tumors, polyps or other organic diseases, and who were overall healthy with no underlying diseases such as hypertension and diabetes. 2. Bulk RNA-seq data. To investigate the effect of bile acis on the transcriptome of mouse intestinal organoids, mouse small intestinal organoids cultured for 2 days were incubated with a series of bile acids at 50 mmol/L for 96 hours and lysed for RNA extraction. 3. ScRNA-seq experiments in mouse duodenum organoids stimulated with DCA (50 mmol/L for 96 hours) and DMSO were employed to directly depict the changes in the proportion of epithelial lineages.

本数据集提供了新生成的单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）与批量RNA测序（bulk RNA-seq）数据，具体如下：1. 单细胞RNA测序（scRNA-seq）数据：为探究人类肠道的区域异质性，本研究从10名健康志愿者中采集了14份十二指肠、空肠及回肠活检样本；这些志愿者均因接受常规胃镜、小肠镜或结肠镜检查入组，且无胃肠道肿瘤、息肉或其他器质性疾病，同时未患有高血压、糖尿病等基础疾病，随后对样本开展单细胞RNA测序。2. 批量RNA测序（bulk RNA-seq）数据：为探究胆汁酸对小鼠肠道类器官转录组的影响，本研究将体外培养2天的小鼠小肠类器官置于浓度为50 mmol/L的系列胆汁酸中孵育96小时，之后裂解样本并提取RNA，进行批量RNA测序。3. 小鼠十二指肠类器官scRNA-seq实验数据：本研究设置了脱氧胆酸（DCA，50 mmol/L，孵育96小时）刺激组与二甲基亚砜（DMSO）对照组，通过该实验直接刻画上皮细胞谱系比例的变化情况。