Gene loci fluctuations reveal bacterial chromosome dynamical organization

Chromosomal sites in rod-like bacteria move sub-diffusely, driven by the out-of-equilibrium nature of the viscoelastic, crowded intracellular environment. Furthermore, it has been shown that there is a pronounced dynamical asymmetry between longitudinal (long-axis) and transverse (radial) motions, manifested in different exponents in the mean square displacements, and respectively. Here, using polymer simulations and experimental observations of a locus in Bacillus subtilis, we substantiate the notion that asymmetric dynamics are a result of the asymmetric structure of the bacterial chromosome modelled as a bottlebrush polymer in a poor solvent. Our simulations recapitulate the observed asymmetry as well as the range of the observed exponent values that are related to scaling models of polymer dynamics, lending support to the notion that asymmetric dynamics is a consequence of the bottlebrush structure of the bacterial chromosome., , # Gene loci fluctuations reveal bacterial chromosome dynamical organization

Dataset DOI: [10.5061/dryad.g1jwstr4d](10.5061/dryad.g1jwstr4d)

## Description of the data and file structure

### Description of the data and file structure

Deposited data includes the following data files used to generate different figures in the main text, with their respective description (in Origin, Notepad and Excel files).

## File: Data_Fig1.opju

**Description:** This dataset contains experimental raw data of the anisotropic mean square displacement (MSD) of a chromosomal locus as a function of time. The measurements were obtained from time-lapse microscopy tracking in the image plane.

##### File structure:

The data are organized into three worksheets of an Origin file corresponding to different displacement components:

* MSDX — Mean square displacement in the transversal (x) direction
* MSDY — Mean square displacement in the longitudinal (y) direction
* MSDr — Mean square displacement in the radi...,

杆状细菌的染色体位点呈现亚扩散运动，该运动由粘弹性且拥挤的细胞内环境的非平衡特性驱动。已有研究表明，纵向（长轴方向）与横向（径向）运动之间存在显著的动力学不对称性，该不对称性体现为二者的均方位移（mean square displacement, MSD）具有不同的指数特征。本研究借助聚合物模拟以及枯草芽孢杆菌（Bacillus subtilis）某基因位点的实验观测结果，证实了动力学不对称性源于细菌染色体的不对称结构——该染色体可被建模为不良溶剂中的刷状聚合物（bottlebrush polymer）。我们的模拟结果重现了实验观测到的不对称性，以及与聚合物动力学标度模型相关的指数取值范围，进一步支持了“动力学不对称性是细菌染色体刷状结构的必然结果”这一观点。

# 基因位点波动揭示细菌染色体的动态组织

数据集DOI: [10.5061/dryad.g1jwstr4d]

## 数据与文件结构说明

### 数据与文件结构说明

存档数据包含用于生成正文各图的相关数据文件，附带各自的说明文档（以Origin、记事本及Excel文件形式存储）。

## 文件: Data_Fig1.opju

**说明：** 该数据集包含染色体位点各向异性均方位移（mean square displacement, MSD）随时间变化的实验原始数据。实验数据通过图像平面内的延时显微镜追踪获取。

##### 文件结构：
该数据存储于Origin文件的三个工作表中，分别对应不同的位移分量：
* MSDX —— 横向（x方向）均方位移
* MSDY —— 纵向（y方向）均方位移
* MSDr —— Mean square displacement in the radi...,