Investigation of the Impact of Microbial Communities and Soil Physicochemical Properties on the Successful Cultivation of Morchella in Greenhouses(ITS sequence)

In April 2024, at the morel mushroom (M. sextelata) cultivation park located in Shuangcheng District, Harbin City, Heilongjiang Province, China , certain greenhouses exhibited either dead or absent mushroom growth (Figure 1). To investigate the causes of these issues, a five-point sampling method was employed to collect surface soil (1-5 cm) from within the same greenhouse, which were then combined into a single sample. The soil samples from each location were sieved through a 2 mm sieve, with roots and plant debris manually removed. Subsequently, the sample was divided into two parts: one was air-dried for soil physicochemical property determination, and the other was rapidly placed in liquid nitrogen, transported to the laboratory, and stored in a -80 Celsius freezer for subsequent DNA extraction. A total of nine soil samples were collected, comprising successfully fruiting samples (CK, 3 samples), dead mushroom samples (DM, 3 samples), and unfruitful samples (UM, 3 samples).