Data from: Intruder colour and light environment jointly determine how nesting male stickleback respond to simulated territorial intrusions

AbstractVariation in male nuptial colour signals might be maintained by negative frequency-dependent selection. This can occur if males are more aggressive towards rivals with locally common colour phenotypes. To test this hypothesis, we introduced red or melanic three-dimensional printed-model males into the territories of nesting male stickleback from two optically distinct lakes with different coloured residents. Red-throated models were attacked more in the population with red males, while melanic models were attacked more in the melanic male lake. Aggression against red versus melanic models also varied across a depth gradient within each lake, implying that the local light environment also modulated the strength of negative frequency dependence acting on male nuptial colour., Usage notesAttack countsReadMe details for “Bolnick_data.csv” for Bolnick et al. Biology Letters 2016 The file Bolnick_data.csv is a comma-delimited ..csv text file. This file contains columns necessary to recreate analyses presented in the Biology Letters paper. The data in this table is a synthesis of a raw data file containing the exact time of each interaction, obtained from the original videos that are available upon request from the author (large files). Each column is distinct information regarding individual fish (rows). These columns are: A) Lake: Blackwater Lake or Gosling Lake B) Fish: an ID number corresponding to the camera used to uniquely video tape each interaction C) Model_color: Red, Blue, or Control_object (a cylinder the same size as our models) D) depth: Resident males’ nest depth (cm) E) N_bites: Number of bites directed at the model over a 1 hour video F) N_inspects: Number of inspections of the model (focal fish approaching the model to within one body length while facing the model) G) Aggressive_interactions: Sum of bites and inspections H) Date: date of experiment I) Mean.interval.between.bites: the mean duration of time between successive bites. Not calculated for Blackwater Lake because too few fish conducted more than 2 bites. Measured in seconds. J) Median.interval.between.bites: the median duration of time between successive bites. Not calculated for Blackwater Lake because too few fish conducted more than 2 bites. Measured in seconds. K) SD.interval: the standard deviation of the duration of time between successive bites. Not calculated for Blackwater Lake because too few fish conducted more than 2 bites. Measured in seconds.Bolnick_data.csv

摘要：雄性婚饰色彩信号的变异或许可通过负频率依赖选择（negative frequency-dependent selection）得以维持。当雄性对本地常见色彩表型的同类竞争者表现出更强攻击性时，便可能出现这一维持机制。为验证该假说，我们将红色或黑色的3D打印雄性模型，放入来自两个光学特性迥异且栖息种群色彩不同的湖泊中的筑巢雄性棘鱼（stickleback）的领地内。在雄性体色为红色的种群中，红色喉部的模型更易遭到攻击；而在雄性体色偏黑的湖泊中，黑色模型则更易遭受攻击。针对红色与黑色模型的攻击性还会随各湖泊内的深度梯度发生变化，这表明本地光照环境同样会调控作用于雄性婚色信号的负频率依赖选择强度。

使用说明
攻击计数说明：以下为"Bolnick_data.csv"的详细配套说明，该数据集对应Bolnick等学者2016年发表于《生物学快报》（Biology Letters）的研究成果。
"Bolnick_data.csv"为逗号分隔的CSV文本文件，包含复现该论文中分析所需的全部列字段。本表数据源自原始交互时间记录文件的整合，原始记录提取自实验原始录像；若需获取原始录像文件，可联系作者申请（文件体量较大）。
每一行代表一条独立的实验个体，每一列则对应一项独立的实验信息，各字段说明如下：
A) 湖泊：黑水湖（Blackwater Lake）或高斯林湖（Gosling Lake）
B) 鱼类编号：对应每一次交互录像所使用的摄像头的唯一标识编号
C) 模型色彩：红色（Red）、蓝色（Blue）或对照物体（Control_object，即与实验模型尺寸一致的圆柱体）
D) 巢穴深度：筑巢雄性的巢穴水深，单位：厘米
E) 咬击次数（N_bites）：1小时录像时长内，实验鱼对模型发起的咬击总次数
F) 探查次数（N_inspects）：实验鱼靠近模型至自身1个体长范围内且正对模型的探查行为总次数
G) 攻击交互总次数（Aggressive_interactions）：咬击次数与探查次数之和
H) 实验日期
I) 咬击间隔均值（Mean.interval.between.bites）：连续两次咬击之间的平均时长。因黑水湖样本中仅少数个体完成2次以上咬击，故未计算该指标，单位：秒
J) 咬击间隔中位数（Median.interval.between.bites）：连续两次咬击之间的时长中位数。因黑水湖样本中仅少数个体完成2次以上咬击，故未计算该指标，单位：秒
K) 咬击间隔标准差（SD.interval）：连续两次咬击之间的时长标准差。因黑水湖样本中仅少数个体完成2次以上咬击，故未计算该指标，单位：秒