CISI C57BL/6 mouse coronal sections containing MOp - October 2020 - tissue section 9

This dataset is part of an experiment demonstrating Composite In Situ Imaging (CISI) on whole coronal sections from a bisected mouse brain. The experiment profiled 37 genes through a combination of composite measurements and validation measurements in 12 tissue sections, with the validation measurements varying in each section. Both types of measurements were made using cyclic HCR-FISH, with composite measurements consisting of multiple probes simultaneously staining with the same dye in a given round of imaging. Composite measurements were decompressed to recover predicted images for individual genes, and these were compared with validation images and used for downstream analysis (e.g. cell type clustering).

本数据集为一项演示复合原位成像（Composite In Situ Imaging, CISI）技术的实验的组成部分，该实验针对对半剖开的小鼠大脑全冠状切片开展研究。实验在12个组织切片中，通过复合测量与验证测量相结合的方案，对37个基因进行了表达谱表征，且每个切片的验证测量设置均存在差异。两类测量均采用循环杂交链反应荧光原位杂交（cyclic HCR-FISH）完成：其中复合测量指在单次成像轮次中，使用同种染料同时标记多种探针的实验方式。研究人员对复合测量结果进行解压缩处理，以恢复单个基因的预测成像结果，随后将其与验证成像结果进行比对，并将其应用于下游分析（例如细胞类型聚类）。