Biomarkers and PAHs concentrations in caged and wild oysters, Grande Terre, Breton Sound, Barataria Bay, Sister Lake, 2010-2011.

Biomarkers and PAHs concentrations were measured in caged oysters in May 2010 prior to oil reaching the coastline, and in caged and wild oysters in October 2010 and in 2011 at oiled (Grande Terre) and non-oiled sites (Bay Gardene, Sister Lake, Grand Isle north shore). Hemocyte density, viability, phagocytosis and lysosomal stability were measured in individual oysters. Oysters were then shucked and fragments of gills were used to measure HSP70 stress proteins. Standard tissue sections were processed to assess histopathology. The bulk of the tissue from each oyster was then homogenized and processed to measure Perkinsus marinus infection intensities, calculate condition index and determine PAHs concentrations.

2010年5月，即在原油抵达海岸线之前，研究人员对笼养牡蛎的生物标志物与多环芳烃（PAHs）浓度进行了检测；随后分别于2010年10月与2011年，在受原油污染的格兰德泰尔（Grande Terre）站点，以及未受原油污染的贝加登（Bay Gardene）、姐妹湖（Sister Lake）、格兰德艾尔北岸（Grand Isle north shore）站点，对笼养与野生牡蛎开展了相关检测。针对单只牡蛎，研究人员测定了其血细胞密度、存活率、吞噬活性及溶酶体稳定性；随后将牡蛎去壳，取鳃组织片段用于检测热休克蛋白70（HSP70）应激蛋白的表达水平；制备标准组织切片以评估组织病理学变化；最后将每只牡蛎的剩余大部分组织进行匀浆处理，以此测定帕金虫（Perkinsus marinus）的感染强度、计算健康指数，并检测多环芳烃浓度。