Next Generation Sequencing Transcriptome Quantitative Analysis of Livers of Adult Male Offspring of Obese Female Mice

Obesity during pregnancy is associated with increased risk of non-alcoholic fatty liver disease in the offspring. We used RNA next generation sequencing analysis (HiSeq2000) to have a snapshot of the liver transcriptome in twelve week-old male offspring exposed to maternal obesity and weaned onto an obesogenic diet. Overall design: Thirty-five female C57BL/6J mice were fed either a standard control diet, RM1 [7% simple carbohydrates/3% lipids (w/w)] or an energy-rich obesogenic diet [10% simple carbohydrates/20% lipids (w/w)] plus sweetened condensed milk [55% simple carbohydrates, 8% lipids, 8% protein (w/w), supplemented with mineral and vitamin mix AIN93G]. Female mice were mated, and maintained on their diets until weaning. On post-natal day 21, male offspring were weaned onto either standard chow (RM1) or the obesogenic diet, generating 4 experimental groups: maternal and offspring control diet (CC), maternal control diet and offspring obesogenic diet (CO), maternal obesogenic diet and offspring control diet (OC), maternal and offspring obesogenic diet (OO). At 12 weeks of age, mice were killed by raising on a fasted state. Liver were dissected, and snap frozen. Three high quality RNA samples from each group were randomly selected and subjected to a cDNA library preparation for further mRNA sequencing using TruSeq RNA Library Preparation kit