DArTSeq Genotyping of Provena x 94197A1-9-2-2-2-5 and CDC Boyer x 94197A1-9-2-2-2-5 Recombinant Inbred Lines

The data was used for mapping of QTL for oat crown rust resistance\nLineage: DArTSeq genotyping of oat lines was performed by Diversity Arrays Technology Pty Ltd. (https://www.diversityarrays.com/) (Canberra, Australia) using restriction enzymes PstI and HpaII with markers generated in two formats: SilicoDArT (presence/absence variants) and SNPs. The sequences of the DArT markers were aligned to the reference genome sequence of Avena sativa OT3098 v2 and Sang using BLAST (Altschul et al. 1990), with a threshold of an expected value (E) less than 5e-7 and a sequence identity greater than 70%.

本数据集用于燕麦冠锈病抗性数量性状位点（QTL）的定位研究。
品系基因分型：燕麦品系的DArTSeq基因分型由澳大利亚堪培拉的多样性阵列技术私人有限公司（Diversity Arrays Technology Pty Ltd.，https://www.diversityarrays.com/）完成，实验采用限制性内切酶PstI与HpaII进行酶切，生成两种类型的分子标记：SilicoDArT（存在/缺失变异）与单核苷酸多态性（SNPs）。
将上述DArT标记序列与普通燕麦（Avena sativa）参考基因组OT3098 v2及Sang基因组序列进行BLAST比对（Altschul等，1990），比对筛选阈值为期望值（E）小于5e-7且序列相似度高于70%。