Additional file 1: Figure S1. of Lung carcinogenicity of inhaled multi-walled carbon nanotube in rats

Blue arrows indicate the flow of the MWNT-7. Red arrows indicate aerosolization of the MWNT-7 by the upward spiraling airstream. MWNT-7 (Bulk MWNT-7) is first placed into the dust feeder. The dust feeder transports a portion of the MWNT-7 into the sieving chamber (Left panel). In the sieving chamber, clean air is aspirated from 9 diagonally opened slits by using the ejector air as the driving force, so that an upward spiraling airstream is continuously generated in the cylindrical sieving chamber. MWNT-7 is dispersed and aerosolized by the high-speed spiraling air. Light MWNT-7 particles are carried to the top of the sieving chamber, where a partitioning sieve is located. Only the sieved MWNT-7 can be delivered to the inhalation chamber. Ionizers are used to avoid agglomeration. This equipment has a feedback system for keeping the aerosol concentration in the inhalation chamber constant. (PPS 2292 kb)