Effect of the microstage device on image stability and quality.

(A) Microstage device. (B) Illustration of the microstage device and microstages of three types. (C) Illustration showing that a portion of the pancreas of an anesthetized mouse was positioned on the microstage and imaged using a stick-type water immersion lens. (D) In vivo images of liver vasculature (left, red, labeled with AngioSense 750 IVM) and liver cells (right, green) of the GFP–LC3 mouse taken without (top row) and with (bottom row) the microstage device. Arrows depict GFP-labeled autophagic membranes. Individual images in D are 512×256 pixel areas cropped from 512×512 pixels. Scale bars, 20 µm.