Single-molecule microscopy data related to "Telomeres stall DNA loop extrusion by condensin"

Single-molecule imaging data files of DNA loop extrusion by yeast condensin on DNA with Rap1 protein arrays. Both whole fields of view (FOVs) and single cropped DNA molecules are deposited.<br>The dataset is part of a cell biology and biophysics study of how the process of DNA loop extrusion by condensin interacts with multiple roadblocks on DNA. We found that arrays of proteins may stall DNA loop extrusion dependent on their length and density, both in vivo and in vitro.<br>The data was generated using single-molecule HILO fluorescence microscopy on DNA (intercalating fluorophores) and fluorescently labeled proteins (Rap1 and LacI proteins). This subset of data gives a representative overview of the data that was obtained for the related journal article. It serves as a demonstration of the type of data and the type of events that were analyzed.<br>The README file contains more specific information on the imaging and file structure/naming.

酵母凝缩蛋白（condensin）在带有Rap1蛋白阵列的DNA上执行DNA环挤出实验的单分子成像数据文件已存档，其中包含完整视场（field of view, FOV）与单条裁剪后的DNA分子两类数据。
本数据集隶属于一项细胞生物学与生物物理学研究，该研究聚焦于凝缩蛋白介导的DNA环挤出过程与DNA上多重阻断位点的相互作用机制。研究团队发现，无论在体内（in vivo）还是体外（in vitro）环境中，蛋白阵列均可依据自身长度与密度阻断DNA环挤出过程。
本数据集通过单分子HILO荧光显微镜采集获得，其中DNA通过嵌入型荧光染料（intercalating fluorophores）标记，靶蛋白（Rap1与LacI蛋白）则经荧光标记实现示踪。该数据子集可完整代表相关学术论文所采用的实验数据，用于展示本次研究中分析的数据类型与事件类型。
自述文件（README）中包含关于成像方法、文件结构与命名规则的更多详细信息。