Morphotaxonomy and molecular phylogeny unveil one new species and two new records of Phlegmacium (Cortinariaceae) from India

A macrofungal exploration of two different states of India (Uttarakhand and Sikkim) has revealed interesting agaricus species belonging to the genus Phlegmacium (Cortinariaceae). Phlegmacium didnaense is proposed here as a new species, whereas P. ophiopus and P. largum are reported for the first time from India. All three taxa are morphologically described and with molecular phylogenetic estimations., , , # Morphotaxonomy and molecular phylogeny unveil one new species and two new records of Phlegmacium (Cortinariaceae) from India

[https://doi.org/10.5061/dryad.2fqz612zt](https://doi.org/10.5061/dryad.2fqz612zt)

## Description of the data and file structure

**Sequence alignment and Phylogenetic analysis**

The ITS sequences of the newly described Cortinarius species plus close relatives were retrieved from a nBLAST search against GenBank ([https://www.ncbi.nlm.nih.gov/genbank](https://www.ncbi.nlm.nih.gov/genbank)), UNITE database ([https://unite.ut.ee](https://unite.ut.ee)), BOLD SYSTEMS ([https://boldsystems.org/](https://boldsystems.org/)), and relevant published phylogenies. The ITS dataset was aligned using the online version of the multiple sequence alignment program MAFFT v. 7

([https://mafft.cbrc.jp/alignment/software/](https://mafft.cbrc.jp/alignment/software/)) with the L-INS-i strategy. The alignment was manually checked and trimmed with the conserved motifs in MEGA v. 7.

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对印度北阿坎德邦与锡金邦两个邦开展的大型真菌调查，发现了隶属于肥脚丝膜菌属（Phlegmacium）、丝膜菌科（Cortinariaceae）的若干颇具研究价值的伞菌物种。本研究提出Phlegmacium didnaense为新物种，而P. ophiopus与P. largum为印度境内首次报道的物种。对这三个分类单元均进行了形态学描述，并开展了分子系统发育推断。

# 形态分类与分子系统发育研究揭示印度境内肥脚丝膜菌属（Cortinariaceae）一新种与两新记录种

[https://doi.org/10.5061/dryad.2fqz612zt](https://doi.org/10.5061/dryad.2fqz612zt)

## 数据与文件结构说明

**序列比对与系统发育分析**

本研究通过nBLAST搜索，从基因银行（GenBank，[https://www.ncbi.nlm.nih.gov/genbank](https://www.ncbi.nlm.nih.gov/genbank)）、UNITE数据库（[https://unite.ut.ee](https://unite.ut.ee)）、BOLD系统（[https://boldsystems.org/](https://boldsystems.org/)）及已发表的相关系统发育研究中，获取了新描述的丝膜菌科物种及其近缘类群的内转录间隔区（Internal Transcribed Spacer，ITS）序列。本研究使用在线版多序列比对软件MAFFT v.7（[https://mafft.cbrc.jp/alignment/software/](https://mafft.cbrc.jp/alignment/software/)），采用L-INS-i算法对ITS数据集进行比对。利用MEGA v.7软件，手动核查并修剪比对结果中的保守基序区域。

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