Bacterial phylotypes associated with Lutzomyia sand flies sampled from 4 field sites.
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Insects collected in field locations were suspended in 70% ethanol. DNA extracts from pooled samples of insects were subjected to PCR using primers specific for a 900 bp region of the 16S rRNA gene. The PCR products were cloned and colonies randomly selected. Purified plasmids from the clones were subjected to specific PCR, the products separated with DGGE and representative clones were selected on the basis of the band pattern of their DGGE profiles.
创建时间:
2015-12-02



