Master TFs bind SOEs to drive tumorigenesis in LUSC cells [ChIP-seq]

These SOEs were highly enriched for H3K27ac signals in H520/HCC95 cells. Binding of key TFs, also known as mater regulators, is usually enriched in cell fate determinant or disease-specific cis-regulatory elements, such as super-enhancers.To find out which TFs were essential for the tumorigenesis of LUSC, sequence motif enrichment analysis was performed in SOE using all CDE as background. As expected, the well-established master TFs of LUSC, i.e., SOX2 and TP63, exhibited the highest enrichment of binding sites among all tested TFs .To confirm their binding in SOEs, we performed ChIP-seq for these factors, including p63, SOX2, GRHL2 and KLF5, all of which exhibited significant and specific binding to most SOEs, compared to other non-SOE genomic regions. Overall design: ChIP-seq of the H3K27ac in BEAS-2B, H520, HCC95, H226 cells and TP63, SOX2, GRHL2 and KLF5 in HCC95 cells.