16S rRNA sequences from Siganidae (S. rivulatus and S. luridus) gut microbiome in their native (Red Sea) and invaded (Mediterranean Sea) ranges

The microbiome of invasive species is increasingly seen as a potential key factor of their ecological success, and this appears particularly true in herbivorous invaders whose digestive abilities rely on the microbes hosted in their gut. We characterized the gut microbiome of two invasive herbivorous fishes (Siganus rivulatus and Siganus luridus) in their native (Red Sea) and invaded (Levantine Sea and Northern Crete) ranges. We found that gut bacterial communities contain a higher taxonomic and phylogenetic diversity while becoming increasingly different from the native microbiome as the fishes move away from the native zone. This shift resulted in the homogenization of the microbiomes between individuals from the same species as well as between the two species. Firmicutes and Tenericutes reduced drastically in abundance while Proteobacteria and Bacteroidetes became more dominant in both species. This led to a modification of the functional potential of the gut microbiome associated wi..., Samples collection
Samples were collected in three regions. Both S. rivulatus and S. luridus were sampled in their native range in the Northern Red Sea (Eilat, Israel), as well as their non-native range in the Levantine Sea and the Northern Crete. Two sampling campaigns were led per region, one in late Spring (June 2018 and 2019 in Israel and Crete, respectively) and one in early Autumn (October 2018 and 2019 in Israel and Crete, respectively). In each region, two to four distrinct sites were selected to cover the various types of habitat structure and macrophytes communities observed locally.
Fish were collected in shallow habitats (2-10 m) by scuba divers using handnets and gillnets (fishing permit n° B13663). Only adult individuals with a standard length > 100 mm were collected. Fish were immediately euthanized, stored on ice and processed less than two hours after their capture. Fish were sized (mm), weighted (g) and dissected using tools cleaned with 70° ethanol. The last third..., , # 16S rRNA sequences from Siganidae (S. rivulatus and S. luridus) gut microbiome in their native (Red Sea) and invaded (Mediterranean Sea) ranges

The dataset contains the sequences and metadata used to describe the gut microbiome of mediterranean Siganidae in their native and invaded range.

## Description of the data and file structure

The file entitled siganidae_microbiome_16S_sequences.zip contains the raw sequences obtained from sequencing of the bacterial 16S rRNA gene amplified from the gut content and the environment of Siganidae in their native and Mediterranean range.

The file table_metadata.csv contains the metadata associated with the sequences. It contains the following columns:

* sample_id_fastq: the id of the sample in the siganidae_microbiome_16S_sequences.zip file
* barcoding_id: the id of the samples used for barcoding analyses
* sample_id: the unique sample ID
* sampling_id: the ID of the sampling event
* taxonomy: the taxonomic identification of the sa...

外来入侵物种的肠道微生物组（gut microbiome）愈发被视为其生态成功的潜在关键因素，这一点在食草性入侵物种中尤为显著——这类入侵者的消化能力依赖于肠道内定殖的微生物。本研究对两种入侵草食性鱼类：黄斑蓝子鱼（Siganus rivulatus）和褐蓝子鱼（Siganus luridus）在其自然分布区（红海）与入侵分布区（黎凡特海与克里特岛北部）的肠道微生物组进行了表征。研究发现，随着鱼类远离其自然分布区，其肠道细菌群落的分类学与系统发育多样性更高，且与本土微生物组的差异愈发显著。这种变化使得同一物种个体间以及两个物种间的微生物组趋于均质化。厚壁菌门（Firmicutes）与柔膜菌门（Tenericutes）的丰度大幅降低，而变形菌门（Proteobacteria）和拟杆菌门（Bacteroidetes）在两个物种中均占据更主导的地位，进而改变了肠道微生物组的功能潜能……

### 样本采集
样本采集于三个区域。研究人员在其自然分布区红海北部（以色列埃拉特）以及入侵分布区黎凡特海与克里特岛北部，对黄斑蓝子鱼和褐蓝子鱼进行了采样。每个区域开展两次采样活动：一次在晚春（以色列与克里特岛分别于2018年6月、2019年6月），另一次在初秋（以色列与克里特岛分别于2018年10月、2019年10月）。每个区域选取2至4处独立样点，以覆盖当地观测到的各类生境结构与大型藻类群落。
研究人员通过水肺潜水员使用手网与刺网在浅海生境（2-10米）采集鱼类（捕捞许可编号：B13663），仅选取标准体长大于100毫米的成年个体。捕获后的鱼类立即进行安乐死，置于冰上保存，并在捕获后两小时内完成处理。对鱼类进行体长（毫米）、体重（克）测量后，使用经70%乙醇消毒的工具进行解剖。取肠道最后三分之一段……

### 蓝子鱼（黄斑蓝子鱼与褐蓝子鱼）原生与入侵地中海分布区肠道微生物组的16S核糖体RNA（16S rRNA）序列
本数据集包含用于描述原生与入侵分布区地中海蓝子鱼肠道微生物组的序列与元数据。

#### 数据与文件结构说明
名为siganidae_microbiome_16S_sequences.zip的压缩包包含从蓝子鱼肠道内容物及其生境中扩增得到的细菌16S核糖体RNA（16S rRNA）基因测序所得的原始序列。
table_metadata.csv文件包含与序列相关的元数据，其包含以下列：
* sample_id_fastq：对应siganidae_microbiome_16S_sequences.zip文件中的样本ID
* barcoding_id：用于条形码分析的样本ID
* sample_id：唯一样本标识符
* sampling_id：采样事件ID
* taxonomy：样本的分类学鉴定信息……