PRODUCTION OF CELLULOLYTIC ENZYMES BY Gelatoporia subvermispora USING DIFFERENT SUBSTRATES

Abstract In this study the solid-state fermentation for production of cellulolytic enzymes by the fungus Gelatoporia subvermispora was optimized. The enzyme activities on filter paper, exo-cellulase, endo-cellulase and xylanase were determined. A Plackett-Burman design (PB) was used to determine the most significant variables (moisture content of substrate, inoculum density, corn steep liquor concentration, pH and peptone concentration) in the enzyme production for each substrate (sugarcane bagasse, sewage sludge and rice straw). The highest value for filter paper activity was obtained using sugarcane bagasse as substrate. The sewage sludge was an excellent medium for the production of xylanase and exo-cellulases. The endo-cellulase activity was similar in all substrates tested. This is the first report of the Gelatoporia genus for the production of cellulolytic enzymes, being a promising strain for this purpose.

摘要 本研究对利用亚蠕孢胶孔菌（Gelatoporia subvermispora）生产纤维素酶的固态发酵工艺进行了优化。测定了该菌株的滤纸酶活、外切纤维素酶活性、内切纤维素酶活性及木聚糖酶活性。采用普拉凯特-伯曼试验设计（Plackett-Burman design，PB），针对甘蔗渣、污泥与稻草秸秆三种底物，分别筛选出产酶过程中的关键显著变量：底物含水率、接种密度、玉米浆浓度、初始pH值及蛋白胨浓度。以甘蔗渣为底物时，滤纸酶活达到最高水平。污泥是生产木聚糖酶与外切纤维素酶的优质培养基。在所测试的全部底物中，内切纤维素酶活性均无显著差异。本研究首次报道了胶孔菌属（Gelatoporia）真菌用于纤维素酶生产，该菌株具备良好的纤维素酶生产应用潜力。