Additional file 1 of Effects of in ovo delivered xylo- and mannan- oligosaccharides on growth performance, intestinal immunity, cecal short-chain fatty acids, and cecal microbiota of broilers

Additional file 1 Table S1 Spearman’s correlation between growth performance parameters and major short-chain fatty acids in each treatment in broilers at d 28 post-hatch. Fig. S1 Biplot (individual samples scatter removed) from principal component (PC) analysis of growth performance parameters and the major cecal short-chain fatty acids showing correlations among these variables in different treatment groups in two PC (PC 1 and PC 2). The PC percentage on the x and y-axis indicates the proportions of the variability of the data explained by those components. The angle between the loading arrows and their directions indicates negative, positive, or no association, while the length indicates the value of correlation (e.g., α = 0° and r = 1; α = 90° and r = 0; and α = 180° and r = − 1). See Table S1 for the significant spearman’s correlation coefficient. ADFI: average daily feed intake, ADG: average daily gain, FCR: feed conversion ratio, FBW: final body weight. NSC: normal saline, XOS3: xylotriose, XOS4: xylotetraose, MOS3: mannotriose, MOS4: mannotetraose, NIC: no injection control.

附加文件1 表S1：孵化后28日龄肉鸡各处理组生长性能参数与主要短链脂肪酸（short-chain fatty acids）的斯皮尔曼（Spearman’s correlation）相关性分析。
图S1：针对生长性能参数及主要盲肠短链脂肪酸开展主成分（PC）分析所得的双标图（已移除个体样本散点），展示不同处理组中各变量在主成分1（PC1）与主成分2（PC2）上的相关性。横纵坐标轴标注的PC百分比代表对应主成分对数据总变异的解释比例。载荷箭头间的夹角与指向可反映变量间的负相关、正相关或无关联关系，箭头长度则代表相关系数的大小（例如，夹角α=0°且r=1表示完全正相关；α=90°且r=0表示无关联；α=180°且r=-1表示完全负相关）。显著斯皮尔曼相关系数详见表S1。
术语说明：ADFI：平均日采食量（average daily feed intake），ADG：平均日增重（average daily gain），FCR：饲料转化率（feed conversion ratio），FBW：终末体重（final body weight）；NSC：生理盐水（normal saline），XOS3：木三糖（xylotriose），XOS4：木四糖（xylotetraose），MOS3：甘露三糖（mannotriose），MOS4：甘露四糖（mannotetraose），NIC：无注射对照组（no injection control）。