Pacific herring HIF-1a

A hypoxia-specific responsive gene, hypoxia-inducible factor-1α (HIF-1α), was identified and tested as a potential biomarker for hypoxia exposure in Pacific Herring <em>Clupea pallasii</em>. <em>Lab experiments zip:</em> 'Threshold_data.CSV' HIF-1a expression data from Theshold experiement 'Timecourse_data.CSV' HIF-1a expression data from Time-Course experiement 'Mean_threshold_plot.CSV' Mean HIF-1a expression and SE from Threshold experiment for Fig. 5 'Mean_timecourse_plot.CSV' Mean HIF-1a expression and SE from Time-Course experiment for Fig. 6 'ANOVA_Threshold.R' code for two-way ANOVA and Fig.5 'ANOVA_Timecourse.R' code for two-way ANOVA and Fig.6 <em>Field data zip:</em> 'All_field_qPCR.CSV' is the HIF-1a expression from the field and binary data identifying significantly elevated levels of expression. 'GLM_Field.R' is the code for the generalized linear models run on the binary field data.

本研究鉴定并测试了缺氧特异性响应基因——缺氧诱导因子-1α（hypoxia-inducible factor-1α，HIF-1α），将其作为太平洋鲱（*Clupea pallasii*）缺氧暴露的潜在生物标志物。
<em>实验室数据集压缩包：</em>
'Threshold_data.CSV'：阈值实验中的HIF-1α表达量数据
'Timecourse_data.CSV'：时间进程实验中的HIF-1α表达量数据
'Mean_threshold_plot.CSV'：阈值实验中HIF-1α的平均表达量及标准误数据，用于绘制图5
'Mean_timecourse_plot.CSV'：时间进程实验中HIF-1α的平均表达量及标准误数据，用于绘制图6
'ANOVA_Threshold.R'：用于双因素方差分析及绘制图5的R代码
'ANOVA_Timecourse.R'：用于双因素方差分析及绘制图6的R代码
<em>野外数据集压缩包：</em>
'All_field_qPCR.CSV'：包含野外样本的HIF-1α表达量数据，以及用于识别表达量显著上调的二分类数据
'GLM_Field.R'：针对二分类野外数据构建广义线性模型的R代码