Influence of perfluorocarbons on protein adsorption and adsorbates

We propose a combined grazing incidence X-ray diffraction and X-ray reflectivity study on the effect of aerolized and gaseous perfluorocarbons (a- and gFCs) to protein adsorption at Langmuir films using different proteins, surface pressures and gas pressures at beamline ID10 of the ESRF. Aim of the proposed experiment is the determination of the lateral and vertical structure of model membranes of lung surfactant lipids like anionic DPPA and zwitterionic DPPC before and after protein adsorption and the influence of an overlying FC atmosphere to the protein adsorption behaviour. The substances have been selected based on their biomedical research interest. Especially a- and aFCs with a high vapour pressure are in focus of research as they represent a new approach to replace liquid FC ventilation and the unsafe use of the animal LS replacements to treat LS injuries or deficiency in adults and neonatal children. The combination of XRR and GID gives a comprehensive picture of the system.

本研究拟在欧洲同步辐射光源（ESRF）的ID10光束线上，针对雾化全氟碳化物（a-FCs）与气态全氟碳化物（g-FCs）对朗缪尔膜表面蛋白质吸附的影响，开展掠入射X射线衍射（Grazing Incidence X-ray Diffraction, GID）与X射线反射率（X-ray Reflectivity, XRR）的联合表征实验，实验中将采用不同蛋白质、表面压及气体压力参数。本实验的核心目标为：测定肺表面活性物质脂质模型膜（包括阴离子型二棕榈酰磷脂酸（DPPA）与两性离子型二棕榈酰磷脂酰胆碱（DPPC））在蛋白质吸附前后的横向与纵向结构，并探究上层全氟碳化物气氛对蛋白质吸附行为的影响。所选研究体系基于其生物医学研究价值，其中高蒸气压的雾化与气态全氟碳化物为当前研究热点，因其为替代液态全氟碳通气疗法、以及替代不安全的动物源肺表面活性物质（LS）替代品以治疗成人与新生儿肺表面活性物质损伤或缺乏症提供了全新思路。X射线反射率与掠入射X射线衍射的联合测试可完整呈现该体系的全貌。