Role of Dot6 in size control in Candida albicansSupplementary data revision

<br><b>Figure S1. </b><b>A <i>sch9 </i>mutant adjusts cell size in response to different carbon sources.</b><b></b>Size distribution of log-phase cultures of the indicated WT (CAI4) and <i>sch9</i>strains grown in synthetic glucose (black curve) and glycerol (red) medium.<b><br></b><b>Figure S2. Localization of Dot6-GFP in the vacuole when TOR pathway is compromised. </b>Dot6-GFP fluorescence was visualized using confocal microscopy in cells treated with the TOR pathway inhibitor, rapamycin. Vacuoles were stained using CellTracker Blue CMACdye. Red and blue arrows indicate Dot6-GFP florescence in the vacuole and the nucleus, respectively.<b> </b><b><br></b><b>Table S1. </b>Strains used in the current study and their genotypes.<b><br></b><b>Table S2</b>. Primer sequences used in the current study.<b><br></b><b>Table S3</b>. Gene Set Enrichment Analysis (GSEA) of dot6 mutant transcriptome <b><br></b><b>Table S4</b>. Transcripts differentially expressed in dot6 mutant using a 1.5-fold change cut-off and a 5% false discovery rate.<br><b>Table S5. </b>Functional characterisation of downregulated transcripts in <i>dot6 </i>mutant.<br>

**补充图S1. *sch9* 突变体可响应不同碳源调节细胞尺寸**
指定的野生型（WT, CAI4）及*sch9*菌株在合成葡萄糖（黑色曲线）与甘油（红色曲线）培养基中培养至对数生长期后的细胞尺寸分布。

**补充图S2. TOR（雷帕霉素靶蛋白）通路受抑制时Dot6-GFP的液泡定位**
采用共聚焦显微镜对经TOR通路抑制剂雷帕霉素处理的细胞进行Dot6-GFP荧光成像。使用CellTracker Blue CMAC染料对液泡进行染色。红色箭头与蓝色箭头分别指示液泡与细胞核内的Dot6-GFP荧光信号。

**补充表S1. 本研究使用的菌株及其基因型**

**补充表S2. 本研究使用的引物序列**

**补充表S3. *dot6*突变体转录组的基因集富集分析（GSEA）**

**补充表S4. 以1.5倍变化为阈值、5%错误发现率筛选得到的*dot6*突变体差异表达转录本**

**补充表S5. *dot6*突变体中下调转录本的功能注释**