SH1042182.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为界定、识别、交流以及处理基于DNA的物种假说（Species Hypotheses，以下简称SH）提供了统一的解决方案。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，通过应用一组动态距离阈值（0.5%至3.0%区间），被聚类至近似物种水平的集群。所有物种假说均会被赋予以数字对象标识符（Digital Object Identifier，DOI）为格式的唯一稳定名称，其分类学与生态学注释将通过分布式的网络第三方注释工作完成验证。SH将尽可能关联至对应的分类单元名称及其分类层级（门、纲、目等），关联依据为该SH下所有序列的鉴定结果。研究人员会为每个SH选取一条自动或人工指定的代表序列。此类代表序列已发布于https://unite.ut.ee/repository.php，可供科学界开展局部序列相似性搜索、下一代测序分析流程等研究工作使用。随着公共真菌ITS序列数据量的增长，该系统与数据集将自动进行更新。