Overview of the genotype and first-cycle phenotype of EAV nsp1 mutants described in this study.

aNucleotide substitutions are indicated in bold italics; numbers indicate the start and end coordinates of the mutated codons in the EAV genome.
bTransfected cells were analyzed by IFA and gel hybridization analysis at 11 h post-transfection. Wild-type levels of replication and transcription (based on accumulation levels of genomic and sg mRNAs, respectively) are indicated with +++. A 2- to 4-fold increase in genomic RNA levels as compared to wild-type is denoted by ++++, while an increase of >4-fold is shown as +++++. Likewise, a >2-fold increase or decrease in accumulation levels of at least two sg mRNA species is shown as ++++ and ++, respectively (see text for details).
cNA, not applicable.