Comparisons between fold-change in gene expression determined by microarray and qRT-PCR methods.

The fold changes in mRNA levels in diabetes (DM) and DM+SERCA2a samples of selected genes were determined by microarray and RT-qPCR. Transcripts were selected based on their roles in insulin signaling, energy/metabolism, Ca2+ handling, structural remodeling and intracellular signaling. CK, creatine kinase; sFRP4, secreted frizzled-related protein 4; Retnla, resistin-like alpha; Cacna1c, calcium channel, voltage-dependent, L type, alpha 1C subunit; Cacnb3, calcium channel, voltage-dependent, beta 3 subunit. N/A, not amplified.