RNA Sequencing of C elegans wild type (N2) grown on 20 vs 5µg/ml cholesterol

Purpose: Cholesterol is an essential nutrient for diverse biological processes in all organisms. However, the role of cholesterol in immune function remains understudied. Hence the goal is to obtain cholesterol-mediated immune genes Methods: Hermaphrodite C. elegans (var. Bristol) wild type (N2) were grown on 20 along side with control, which is 5µg/ml cholesterol and harvested at L4 stage. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analyses of the transcriptomic data show that cholesterol-mediated immune gene that could enhance the fight against infectious diseases Conclusions: This study showed a novel role for cholesterol in the enhancement of the innate C. elegans RNA seq of L4 grown on 20 and 5µg/ml cholesterol were generated by deep sequencing, in triplicate, using BGISEQ-500 platform