Urinary signatures are associated with calorie restriction-mediated weight loss in obese Diversity Outbred mice

Metabolomic profiles are increasingly being used to identify responders to dietary interventions. Advances using this approach are particularly needed to personalize and enhance the effectiveness of dietary weight loss interventions. Using obese Diversity Outbred (DO) mice that model genetic and phenotypic heterogeneity of human populations, we aimed to identify urinary metabolite signatures associated with responsiveness to calorie restriction (CR)-mediated weight loss. DO mice (150 males, 150 females) were fed a high-fat diet for 12 weeks to induce obesity, then urine was collected and an 8-week CR regimen (30 % decrease in energy intake) initiated. At study completion, mice were rank-ordered according to their percent body weight change, with mice in the extreme quartiles deemed CR responders (n = 67) versus nonresponders (n = 67). Targeted semi-quantitative metabolomics identified elevated glutamic acid and hydroxyproline as key urinary metabolites that distinguish CR responders fro..., , # Data from: Urinary signatures are associated with calorie restriction-mediated weight loss in obese Diversity Outbred mice

Dataset DOI: [10.5061/dryad.8931zcs4n](https://doi.org/10.5061/dryad.8931zcs4n)

## Description of the data and file structure

Targeted semiquantitative metabolomics from biobanked urine samples from obese calorie restriction responders (CR-Responders) and calorie restriction nonresponders (CR-Nonresponders) Diversity Outbred mice collected immediately before the calorie restriction intervention.

### Files and variables

#### File: All_CR-R_vs_CR-NR.csv

**Description:** Urinary metabolite concentrations of male and female CR-responders and CR-nonresponders.

##### Variables

* ID: M_#
* CR-responder: Calorie restriction responder
* CR-nonresponder: Calorie restriction nonresponder
* NA: Missing (not detected)

#### File: Female_CR-R_vs_CR-NR.csv

**Description:** 

##### Variables

* **Description:** Urinary metabolite concentrations of female CR-responders an...,

代谢组学特征正日益被用于识别膳食干预的响应者。当前亟需通过该方法的进展来实现膳食减重干预的个性化并提升其有效性。本研究以模拟人类人群遗传与表型异质性的肥胖型多样性远交（Diversity Outbred, DO）小鼠为模型，旨在鉴定与热量限制（calorie restriction, CR）介导减重响应相关的尿液代谢特征。

我们共纳入150只雄性、150只雌性DO小鼠，先以高脂饲料喂养12周以诱导肥胖，随后收集尿液并启动为期8周的热量限制方案（能量摄入降低30%）。实验结束后，根据小鼠体质量变化百分比进行排序，将处于极端四分位组的小鼠划分为热量限制响应者（n=67）与非响应者（n=67）。靶向半定量代谢组学分析发现，谷氨酸与羟脯氨酸升高可作为区分热量限制响应者与非响应者的关键尿液代谢物……

# 数据来自：《肥胖型多样性远交小鼠中尿液特征与热量限制介导减重的相关性》
数据集DOI：[10.5061/dryad.8931zcs4n](https://doi.org/10.5061/dryad.8931zcs4n)

## 数据与文件结构说明
本数据集包含来自肥胖型热量限制响应者（CR-响应者）与非响应者（CR-非响应者）DO小鼠生物库尿液样本的靶向半定量代谢组学数据，样本采集于热量限制干预启动前即刻。

### 文件与变量说明
#### 文件：All_CR-R_vs_CR-NR.csv
**描述：** 雄性与雌性CR-响应者、CR-非响应者的尿液代谢物浓度数据。
##### 变量说明：
* ID：格式为M_#
* CR-responder：热量限制响应者
* CR-nonresponder：热量限制非响应者
* NA：缺失值（未检出）

#### 文件：Female_CR-R_vs_CR-NR.csv
**描述：**
##### 变量说明：
* **描述：** 雌性CR-响应者与……