LipidSearch Dataset for Lipidomics in: Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat with tunable lipid compositions for food applications

Included here are the datasets received from the Mass Spectrometry Facility (John Asara Laboratory) at the Beth Israel Deaconess Medical Center in Boston, Massachusetts, United States. The datasets are the peak aligned and lipid species-identified LC-MS/MS data output from LipidSearch (ThermoFisher). Samples of in vitro and in vivo adipose tissue were lipid extracted using methyl tert-butyl ether (MTBE) and dried under nitrogen, then submitted to the John Asara Laboratory at the Beth Israel Deaconess Medical Center. LC-MS/MS (lipidomics) was performed on the samples in accordance with Breitkopf et al. 2017. (https://link.springer.com/article/10.1007/s11306-016-1157-8) In our paper \"Aggregating in vitro-grown Adipocytes to Produce Macroscale Cell-Cultured Fat with Tunable Lipid Compositions for Food Applications\", we processed these datasets to quantify the triacylglyceride and phospholipid contents of our various adipose samples in accordance with https://github.com/mksaad28/Yuen_et_al This dataset includes data on other lipids that are not triacylglycerides/phospholipids. The dataset additionally includes lipidomics data of commercially bought beef and chicken fat, which were not used in our paper.

本数据集包含从美国马萨诸塞州波士顿贝斯以色列女执事医疗中心质谱设施（John Asara实验室）获取的全部实验数据。该数据集为通过赛默飞世尔（ThermoFisher）LipidSearch软件处理得到的峰对齐且完成脂质物种鉴定的液相色谱-串联质谱（LC-MS/MS）数据。本研究中的体外（in vitro）及体内（in vivo）脂肪组织样本，均采用甲基叔丁基醚（MTBE）进行脂质提取，经氮气吹干后提交至上述John Asara实验室。本批次样本的液相色谱-串联质谱（LC-MS/MS，脂质组学）检测严格遵循Breitkopf等人2017年发表的实验方案（https://link.springer.com/article/10.1007/s11306-016-1157-8）。在本团队发表的题为《聚集体外培养脂肪细胞以制备可调控脂质组成的宏规模细胞培养脂肪用于食品应用》的论文中，我们依据https://github.com/mksaad28/Yuen_et_al提供的分析流程，对本数据集进行处理，以定量各类脂肪组织样本中的三酰甘油与磷脂含量。本数据集同时涵盖了非三酰甘油/磷脂类的其他脂质相关数据。此外，本数据集还包含市售牛肉脂肪与鸡肉脂肪的脂质组学数据，该部分数据未在本论文中使用。