Raw data supporting “Data set of the antioxidant properties of ascorbyl 2,6-dipalmitate stabilised nanoemulsions and their facilitated mucodiffusion due to the intestinal-protein corona”

Colloidal carriers have greatly contributed to overcome biopharmaceutical limitations of drugs with low oral bioavailability, a fact that is related to their physicochemical properties and composition that determine their biological behaviour. In this sense, we have developed α-tocopherol nanoemulsions stabilised by ascorbyl 2,6-dipalmitate (ADP), a hydrophobic derivative of the ascorbic acid, obtaining the formulations NEE1-ADP, NEE2-ADP and NEE3-ADP. In this repository we collect data supporting the document “Data set of the antioxidant properties of ascorbyl 2,6-dipalmitate stabilised nanoemulsions and their facilitated mucodiffusion due to the intestinal-protein corona”, submitted to Data in brief (November 2019). That data set includes results of the antioxidant properties of NEE-ADP formulations, their stability under saline conditions and the mucodiffusion of uncoated and intestinal-protein coated NEE-ADP nanosystems. The raw data of those experiments are included in this repository record. i) Raw data related to ABTS and DPPH assays (please see folder “raw data ABTS and DPPH assays”). Concretely, we have collected the absorbance values measured at 734 nm (ABTS assay) or at 515 nm (DPPH assay). This was the basis of results further detailed in the mentioned dataset (figures 2 and 3). ii) Raw data related to the ccc determination, showing results of the hydrodynamic mean size measurements carried out at increasing concentrations of CaCl2. These data, which can be found in the folder “raw data ccc determination”, were further analysed to obtain figure 4 included in the mentioned data set. iii) Raw data from particle tracking experiments (figure 5 in the related data set). In these studies, raw data consists on time-lapse videos. Samples analysis required 20 videos but we include a selection of 2 videos per sample due to the high size of the files. Please see folder “raw data Particle Tracking experiments” for further details. These videos show the interaction of NEE1-ADP and NEE3-ADP with intestinal porcine mucus, used as model of the human intestinal mucus (sub-folders “Videos NEE1-ADP (nanoemulsion)” and “Videos NEE3-ADP (nanoemulsion)”. Furthermore, we evaluated the effect of the intestinal protein corona in this process (sub-folders “Videos NEE1-ADP (protein-coated)” and “Videos NEE3-ADP (protein-coated)”).

胶体载体在克服口服生物利用度低的药物的生物制药局限性方面发挥了重要作用，这与其决定其生物学行为的理化性质与组成密切相关。在此背景下，本研究开发了由抗坏血酸疏水衍生物抗坏血酸2,6-二棕榈酸酯（ascorbyl 2,6-dipalmitate，ADP）稳定的α-生育酚纳米乳液，得到NEE1-ADP、NEE2-ADP与NEE3-ADP三种制剂。
本数据集仓库收录了支撑投稿至《Data in brief》（2019年11月）的论文《抗坏血酸2,6-二棕榈酸酯稳定纳米乳液的抗氧化性能及其因肠蛋白冠介导的黏液扩散促进作用》的相关实验数据。该数据集涵盖了NEE-ADP系列制剂的抗氧化性能、盐环境下的稳定性，以及未包被与肠蛋白包被的NEE-ADP纳米系统的黏液扩散特性相关结果。本仓库已收录上述实验的原始数据：
i) ABTS与DPPH抗氧化实验原始数据：详见"raw data ABTS and DPPH assays"文件夹。本研究采集了ABTS实验在734 nm处、DPPH实验在515 nm处测得的吸光度值，该数据为前述论文中详细阐述的实验结果（对应图2与图3）提供了基础支撑。
ii) 临界凝聚浓度（CCC）测定原始数据：收录了不同氯化钙浓度梯度下测得的流体动力学平均粒径结果。该数据存放在"raw data ccc determination"文件夹中，经进一步分析后得到了前述论文中的图4。
iii) 粒子追踪实验原始数据：对应前述论文中的图5。本研究的原始数据为延时录像，每个样本的分析需采集20段视频，但因文件体积过大，本仓库仅收录每个样本的2段代表性视频。详细信息详见"raw data Particle Tracking experiments"文件夹。
这些视频展示了NEE1-ADP与NEE3-ADP和猪肠黏液（作为人肠黏液模型）的相互作用，相关视频分别存放在子文件夹"Videos NEE1-ADP (nanoemulsion)"与"Videos NEE3-ADP (nanoemulsion)"中。此外，本研究还评估了肠蛋白冠在该过程中的调控作用，相关视频存放在子文件夹"Videos NEE1-ADP (protein-coated)"与"Videos NEE3-ADP (protein-coated)"中。