Sex-limited diversification of the eye in Heliconius butterflies

Butterflies have evolved an immense diversity in eye organization to support a range of vision-based behaviors, including courtship, oviposition, and foraging. This diversity has been surveyed extensively across the butterfly phylogeny, and here we take a complementary approach to characterize the eye within a group of closely related Heliconius butterflies. Using a combination of immunostaining for different opsins and eyeshine for determining the distribution of light-filtering screening pigments, we identified several sexually dimorphic features of eye organization where male eyes varied and female eyes did not. Ultraviolet (UV) sensitive photoreceptors varied in which of two UV opsins were expressed, including co-expression of both within single photoreceptors, and these differences were consistent with a role in courtship and conspecific identification. Additional differences across species and sex included the distribution of three ommatidial types defined by the expression patter..., This dataset includes a MATLAB file that includes all of the original eyeshine images that were taken along with the analyzed counts. A second MATLAB file includes immunostaining data for all animals included in the study. The original .czi files for each tissue section is also included. See the README.txt for additional details., , , # Data from: Sex-limited diversification of the eye in Heliconius butterflies

Dataset DOI: [10.5061/dryad.5hqbzkh7v](https://doi.org/10.5061/dryad.5hqbzkh7v)

## Description of the data and file structure

We used a combination of epifluorescent microscopy to examine eyeshine and opsin immunohistochemistry to characterize the organization of the eye in a closely related group of Heliconius cydno butterflies.

### Files and variables

#### File: data.zip

**Description:** This zip file includes all data necessary to reproduce analyses completed in the manucript. It includes two folders and two .mat files.

Folder 1: images

This folder includes the original .czi files for all confocal images used for immunohistochemistry against UV1, UV2, and blue opsins.

Folder 2: immunostaining images

This folder includes the processed version of the confocal images used for all analyses.\
Within this folder there is first a set of folders with names indicating butterfly ID. These additionally\
incl...

蝴蝶已演化出极为丰富的眼部组织多样性，以支撑一系列基于视觉的行为，包括求偶、产卵与觅食。这类多样性已在蝴蝶演化树中得到广泛调研，本研究则采用互补性方法，对一组亲缘关系紧密的袖蝶属（Heliconius）蝴蝶的眼部进行特征分析。我们结合针对不同视蛋白（opsins）的免疫染色（immunostaining）技术与用于检测滤光遮蔽色素分布的眼反光（eyeshine）技术，发现了若干眼部组织的性二态特征：雄性眼部存在差异，而雌性眼部则无此类变化。紫外（UV）敏感光感受器在两种UV视蛋白的表达模式上存在差异，甚至包括单个光感受器共表达两种UV视蛋白的情况，这类差异与求偶及物种识别功能相符。跨物种与性别的额外差异包括三种由表达模式定义的小眼类型的分布……本数据集包含两份MATLAB文件：其一收录了所有采集到的原始眼反光图像及分析得到的计数数据；其二收录了本研究中所有受试动物的免疫染色数据。此外，各组织切片的原始.czi文件也一并收录。详细信息请参阅README.txt。

# 数据来源：袖蝶属蝴蝶眼部的性别限制性分化
数据集DOI: [10.5061/dryad.5hqbzkh7v](https://doi.org/10.5061/dryad.5hqbzkh7v)

## 数据与文件结构说明
我们结合落射荧光显微镜（epifluorescent microscopy）检测眼反光与视蛋白免疫组织化学（immunohistochemistry）技术，对一组亲缘关系紧密的红带袖蝶（Heliconius cydno）蝴蝶的眼部组织特征进行解析。

### 文件: data.zip
**描述:** 该压缩包包含复现本研究论文分析所需的全部数据，内含2个文件夹与2个MATLAB格式文件。
文件夹1: images
该文件夹收录了所有针对UV1、UV2及蓝光视蛋白进行免疫组织化学实验所用共聚焦图像的原始.czi文件。
文件夹2: immunostaining images
该文件夹收录了用于所有分析的共聚焦图像的处理版本。该文件夹下首先包含若干以蝴蝶编号命名的子文件夹，此外还包含……