A 3D-printed capillary tube holder for high-throughput chemotaxis assays

Bacterial chemotaxis is an important behavior to study to understand the spatial segregation of species in mixed communities and the assembly of host microbiomes. This is particularly relevant in the rhizosphere, where chemoattraction towards root exudates is an important determinant of plant colonization. However, current methods to screen chemoeffectors are limited in their throughput, creating a barrier to generating comprehensive datasets describing chemotactic profiles for species of interest. Here, we describe a novel, 3D-printed capillary tube holder approach, which enables up to 384 simultaneous capillary tube chemotaxis assays. We optimized and benchmarked our assay using Escherichia coli k12 and Bacillus subtilis 3610 and their known chemoattractants: serine and aspartate. We then tested the threshold concentration of these chemoattractants in our assay and found we could detect chemoattraction towards concentrations spanning multiple orders of magnitude. In this paper, we des..., , # Data from: A 3D-printed capillary tube holder for high-throughput chemotaxis assays

Dataset DOI: [10.5061/dryad.2jm63xt2v](10.5061/dryad.2jm63xt2v)

## Description of the data and file structure

#### File: Supplementary_files_S3.stl

3D printer design file to print the 384-well capillary tube holders used in the publication titled \"A 3D-Printed Capillary Tube Holder for High-Throughput Chemotaxis Assays.\"

## Code/software

Preview app or MeshLab can be used to open and view this design file.
,

细菌趋化性（Bacterial chemotaxis）是解析混合群落内物种空间分布格局与宿主微生物组组装过程的重要研究对象。该过程在根际（rhizosphere）环境中尤为关键，其中针对根系分泌物（root exudates）的趋化作用是植物定殖的重要决定因素。然而，当前用于筛选化学效应物（chemoeffectors）的方法通量有限，为获取目标物种完整趋化特征数据集造成了阻碍。本研究提出一种新型3D打印毛细管支架（3D-printed capillary tube holder）方案，可同时开展至多384组毛细管趋化实验。我们以大肠杆菌K12（Escherichia coli k12）、枯草芽孢杆菌3610（Bacillus subtilis 3610）及其已知趋化剂丝氨酸（serine）与天冬氨酸（aspartate）为对象，对该实验体系进行了优化与性能验证。随后我们在该体系中测试了上述趋化剂的阈值浓度，发现可检测到跨越多个数量级浓度的趋化响应。本文……（原文此处内容截断），# 数据来源：《用于高通量趋化实验的3D打印毛细管支架》

数据集DOI：10.5061/dryad.2jm63xt2v

## 数据与文件结构说明
#### 文件：Supplementary_files_S3.stl
本文件为3D打印用设计文件，用于制作本研究《用于高通量趋化实验的3D打印毛细管支架》一文中使用的384孔毛细管支架。

## 代码与软件
可使用Preview应用或MeshLab打开并查看该设计文件。