Mapping of the BG2 β-gal-specific T_CD4+ response.

To map the BG2 determinant, transgenic T cells were incubated with splenocytes in the presence of overlapping peptides (1 µM) or whole βgal (50 µg/ml). Supernatants were collected for cytokine analysis 48 hr post-stimulation using the CBA kit from BD Biosciences. Only the peptides shown stimulated cytokine production by BG2 cells.