Glucocorticoid effects on mucosal microbiota in healthy dogs and dogs with IBD

Raw data for ileal and colonic mucosal bacterial counts by bacterial FISH probe in healthy dogs and in IBD dogs following treatment with glucocorticoids.The mucosal microbiota from endoscopic biopsies of inflammatory bowel disease (IBD) dogs (n=8) and healthy controls (HC; n=15 dogs) was evaluated by fluorescence in situ hybridization (FISH) targeting the 16S rRNA genes of total bacteria and bacterial species using a seven probe array. We also used immunohistochemical (IHC) techniques to evaluate the impact of steroid therapy on mucosal apical junction protein (AJP) expression in dogs with IBD.

本数据集收录健康犬与经糖皮质激素治疗的炎症性肠病（IBD）犬的回肠、结肠黏膜细菌荧光原位杂交（FISH）探针计数原始数据。本研究对8只炎症性肠病犬及15只健康对照（HC）犬的内镜活检黏膜微生物群，采用靶向总细菌与细菌物种16S rRNA基因的七探针阵列，通过荧光原位杂交（FISH）技术开展评估。此外，本研究还借助免疫组织化学（IHC）技术，探究了类固醇治疗对炎症性肠病犬黏膜顶连接蛋白（AJP）表达的影响。