Gene expression profile at single cell level of bone marrow and brain cells from young adult and aged mouse after chewing diet (CD) and long-term high-fat diet (HFD) feeding

Aging is one of the risk factors for various maladies such as cognitive deficit and frailty, which are exacerbated under obesity. We used single cell RNA sequencing (scRNA-seq) to analyze the alteration in bone marrow and brain after long-term HFD in both young adult and aged mouse. Overall design: Bone marrow and brain cells of 9-month old after 7-month CD (n=3) or HFD (n=3) and 18-month old C57BL/6 mice after 12-month CD (n=3) or HFD (n=3) were isolated and analyzed using scRNAseq.