RAW DATA supporting “FimH of uropathogenic Escherichia coli enhances infection in prostate cells via JAK/STAT signaling”

This repository contains the original raw experimental data that underpin all primary figures and quantitative results presented in the manuscript:<b>“FimH of uropathogenic </b><b><i>Escherichia coli</i></b><b> enhances infection in prostate cells by the modulation of JAK/STAT signaling pathway”</b>.The dataset includes unprocessed primary files corresponding directly to specific figures as follows:<b>Figure 2 – Prostate Cell Infection Imaging</b><b>Folder:</b> <code>FimH rawdata/fig2/0910/</code><br>Original microscopy images of prostate epithelial cells infected with UPEC strains with varied <b>FimH expression</b> (wild-type, deletion, and overexpression). These images support the bacterial adhesion and invasion phenotypes shown in Figure 2.<b>Figure 3 – Quantitative Infection Assays (CFU counts)</b>Raw colony-forming unit (CFU) count data used to quantify intracellular bacterial burden across experimental groups. These data underlie statistical comparisons presented in Figure 3.<b>Figure 4 – Western Blot Analysis of JAK/STAT Signaling</b><b>Folder:</b> <code>FimH WB/figure 4 raw data/20210811-FimH/</code><br>Subfolders include: <code>1-FimH del/</code> and <code>2-FimH overexp/</code>.<br>This contains uncropped TIFF images of immunoblots (e.g., STAT1, IL-6, β-actin loading control) that support the protein expression analyses presented in Figure 4.<b>Figure 5 – Cytokine Quantification (CBA assays)</b>Raw cytokine measurement values (including IL-6 and IFN-γ) from cytometric bead array (CBA) assays. These data support inflammatory mediator analyses shown in Figure 5.<b>Figure 6 – Integrated Signaling Outcomes</b>Combined quantitative datasets derived from CFU counts, cytokine assays, and western blot densitometry used for integrative analysis of how <b>FimH modulates host signaling responses</b>, as shown in Figure 6.<b>Data Integrity Statement:</b><br>All files in this repository represent original, unedited experimental outputs. Processed values and statistical analyses reported in the manuscript were derived directly from these raw data files.

本仓库收录了支撑论文《尿路致病性大肠埃希菌（uropathogenic Escherichia coli, UPEC）FimH通过调控JAK/STAT信号通路增强前列腺细胞感染能力》中所有主图与定量结果的原始未处理实验数据。
本数据集包含与下述各图直接对应的未处理原始文件：

图2——前列腺细胞感染成像
文件夹：`FimH rawdata/fig2/0910/`
包含感染了不同FimH表达水平（野生型、敲除型与过表达型）尿路致病性大肠埃希菌菌株的前列腺上皮细胞的原始显微镜图像，用于支撑图2展示的细菌黏附与侵袭表型。

图3——定量感染实验（菌落形成单位计数）
原始菌落形成单位（colony-forming unit, CFU）计数数据，用于量化各组实验的胞内细菌载量，支撑图3中的统计对比分析。

图4——JAK/STAT信号通路蛋白质免疫印迹（Western Blot, WB）分析
文件夹：`FimH WB/figure 4 raw data/20210811-FimH/`
其子文件夹包括`1-FimH del/`与`2-FimH overexp/`。本部分包含免疫印迹靶标（如STAT1、IL-6、β-actin内参）的未裁剪TIFF图像，用于支撑图4展示的蛋白表达分析。

图5——细胞因子定量（流式微球阵列（cytometric bead array, CBA）实验）
来自流式微球阵列实验的原始细胞因子检测值（包括IL-6与IFN-γ），支撑图5展示的炎症介质分析。

图6——整合信号通路结果
整合了菌落形成单位计数、细胞因子检测与免疫印迹光密度分析的定量数据集，用于整合分析FimH如何调控宿主信号通路响应，对应图6展示的内容。

数据完整性声明：
本仓库中的所有文件均为未经编辑的原始实验产出物。论文中报告的处理后数值与统计分析均直接源自这些原始数据文件。