Genome resequencing of a engineered Saccharomyces cerevisiae strain ST8424 and its UV mutant for the beneficial mutations for improved muconic acid production.. Genome resequencing of mutant for the beneficial mutations for improved muconic acid production

Genome resequencing of a engineered Saccharomyces cerevisiae strain ST8424 and its UV mutant for the beneficial mutations for improved muconic acid production.Details regarding library preparation and sequencing:• Sequencing facility:o NGS Lab at the Novo Nordisk Foundation Center for Biosustainability• Library preparation kit:o KAPA HyperPlus Library Prep Kit (ROCHE)• Indexing kit:o Dual Indexed PentAdapters, Illumina compatible (PentaBase)• Quality control:o Qubit dsDNA HS Assay Kit (Invitrogen); used for quantification of input DNA and sequencing librarieso DNF-473 Standard Sensitivity NGS Fragment Analysis Kit (1 bp - 6000 bp); (Agilent); used to assess the average size of the sequencing libraries• Average sequencing library size:o 405 bp• Sequencing instrument:o NextSeq 500 (Illumina)• Sequencing kit(s):o NextSeq Mid Output v2 Kit (300 Cycles) (Illumina)• Sequencing reads:o 150 base pair paired-end (2x150 bp)• Index (i7 and i5) reads:o 8 bp, dual indexed• Flow cell loading:o 1.05 pM• Sequencing chemistry:o 2-channel sequencing-by-synthesis (SBS) technology (NS)• Use of PhiX control v3:o Yes; added at 2.5 %