GSE48741: Identification of Transcription Factor RPC-1::RPC-1 Binding Regions in Young Adults

Summary: modENCODE_submission_4034 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: N2(official name : N2 genotype : wild type genotype : DR subclone of DB original (Tc1 pattern I) ); Developmental Stage: Young adult; Genotype: wild type; Sex: Hermaphrodite; EXPERIMENTAL FACTORS: Developmental Stage Young adult; Target gene rpc-1; Strain N2(official name : N2 genotype : wild type genotype : DR subclone of DB original (Tc1 pattern I) ); temp (temperature) 20 degree celsius