Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – Del/+-Del/+ - 5197_5212

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.

本研究针对成对陌生个体间的社交互动与交流行为开展评估。针对此类社交行为与超声通讯的录制工作，我们选择以成对个体为研究对象，原因在于当动物近距离互动时，当前无法识别超声发声（ultrasonic vocalizations，USVs）的发声源。因此，我们对来自不同饲养笼、同年龄（14~20周龄）且基因型一致的陌生个体，开展为期47小时（两昼夜）的无干扰成对互动录制。为此，我们将活体小鼠追踪系统（Live Mouse Tracker，插件版本931）与一台CM16/CMPA麦克风（Avisoft Bioacoustics，德国格利尼克）相连，该麦克风连接至Avisoft Ultrasound Gate 416设备，其参数如下：采样率300kHz，16位格式；触发条件：该通道电平；预触发时长1秒；保持时长1秒；有效时长大于0.005秒；触发事件：25~125kHz频段内能量占比2%且熵值小于50%；上述设备均由德国格利尼克的Avisoft Bioacoustics出品。活体小鼠追踪系统（以下简称LMT）与Avisoft设备依据de Chaumont等人2021年发表的实验协议完成同步。每份上传文件均包含来自LMT的SQLite数据库（已完成处理并完成USVs同步），以及一个压缩包，其中包含Avisoft自动录制的全部USVs序列。