Single-cell RNA Sequencing reveals functional difference of ASCL1-expressing and NEUROD1-expressing SCLC cells

Small cell lung cancer (SCLC) is a particularly aggressive, lethal and widely metastatic lung cancer. Surgical specimens of SCLC are rare and difficult to obtain due to their early metastasis. In this study, we generated single cell lung suspensions of SCLC tissues. ScRNA-seq was performed on CD45 and CD31 negative live cells sorted by FACS. Purified cells were clustered to evaluate the cancer cell sub-clusters. To study the mechanism of lung cancer tumorigenesis and progression , we isolated individual cells (CD45/CD31-) from two freshy resected human primary SCLC, single cell RNA-sequencing was performed on the purified cells.