A distinct pre-treatment immune gene signature in lentigo maligna is associated with imiquimod response
收藏doi.org2025-01-09 收录
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http://doi.org/10.17632/2cc3yvvzk4.2
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资源简介：
Raw Nanostring Files.
Total RNA was extracted from FFPE tissue sections adjacent to sections stained by multiplex IHC, using the RNeasy® FFPE Kit (Cat. 73504, QIAGEN GmbH, Hilden, Germany). 10x10um thick sections were used per sample due to the small specimen size of each biopsy. Concentration was determined using Nanodrop™ 8000 Spectrophotometer (Thermofisher Scientific, PA) and 400ng of total RNA run on the nCounter analysis system™ (nanoString™, WA) using the PanCancer Immune Profiling Panel (nanoString™, WA) consisting of 770 genes.
Files include:
- Raw log2 expression matrix
- Sample identifiers
- Raw .RCC files
- RLF file
原始Nanostring文件。
总RNA是从与多标记IHC染色相邻的FFPE组织切片中提取的，使用RNeasy® FFPE试剂盒（货号73504，QIAGEN GmbH，Hilden，德国）。由于每个活检样本的标本尺寸较小，每个样本使用10x10微米厚的切片。RNA浓度使用Nanodrop™ 8000分光光度计（Thermofisher Scientific，PA）测定，并在nCounter分析系统™（nanoString™，WA）上使用包含770个基因的PanCancer免疫分析套件（nanoString™，WA）对400ng的总RNA进行运行。
文件包括：
- 原始log2表达矩阵
- 样本标识符
- 原始.RCC文件
- RLF文件
提供机构：
Mendeley Data
