NSD3-Short Promotes Migration of A549 Lung Cancer Cells

SGC Open Notebook Project to Characterize the HMTase NSD3 Exp025 Objective: In a previous experiment (exp024), we observed an increase in E-cadherin expression in response to knockdown of the short isoform of NSD3. To determine if this change is functionally relevant, we performed wound healing assays to measure any corresponding alteration in the migratory potential of A549 lung epithelial cancer cells.

SGC开放笔记本项目Exp025：组蛋白甲基转移酶（Histone Methyltransferase，HMTase）NSD3表征研究
实验目标：在既往exp024实验中，我们观察到敲低NSD3短亚型可使E-钙粘蛋白（E-cadherin）的表达量上调。为验证该表达变化是否具备功能相关性，我们通过划痕愈合实验（wound healing assay）检测了A549肺上皮癌细胞的迁移潜能是否发生相应改变。