sec10MO embryos show variable gross phenotypes at 3 dpf.

Abnormal gross phenotypes, scored at 3 dpf, included any of the
following: smaller body size, small eyes, edema, and curly tail down
and curly tail up. Lysates isolated from normal- and
abnormal-appearing 3 dpf sec10MO embryos showed similar levels of
zfSec10 knockdown (data not shown). The following Fisher's
exact tests were performed on the proportions of wild-type embryos:
First, examination of the total percentages of wild-type phenotypes
in all three conditions showed that the frequency of wild-type
embryos differed across conditions (p<10-15). Second,
examination of the 15ng sec10MO embryos compared to the 8+8ng
sec10MO embryos showed that the latter yielded a significantly
higher frequency of wild-type embryos (p<0.01).