Additional file 13 of Altered neuronal physiology, development, and function associated with a common chromosome 15 duplication involving CHRNA7

Additional file 13. Comparison of electrophysiological properties of neurons derived in vitro from the UC-M, AP, and UM iPSC models. (.xlsx). Data is shown for (A) all cells (cExNs and cINs), (B) cExNs, and (C) cINs. (A) Data columns for cells of each genotype show the median, first, and third quartiles, and count (# of cells) for each parameter for all cells recorded, by combining data from cExNs and cINs for each genotype. Columns to the right show neuronal cell type-specific data, by combining cExN data or cIN data for all three genotypes. Light red shading denotes Significant Difference by 2-WAY ANOVA on ranks with post hoc comparison by Student-Newman-Keuls method (p < 0.05) with genotype (UC-M : AP : UM) and neuronal cell type (cExN : cIN) as the two factors. (B-C) Values for (B) cExNs or (C) cINs of each genotype are shown. Tan shading denotes Significant Difference by 1-WAY ANOVA on ranks with post hoc comparison by Student-Newman-Keuls method (p < 0.05), with darker tan indicating that one of the genotypes was different from the other two and lighter tan indicating that two of the three genotypes were different from each other. Capacitance and input resistance were determined from 10 mV voltage steps from -80 mV. First spike threshold, amplitude and half-width were determined for the first action potential recorded at threshold. Maximum first frequency and maximum average firing frequency were determined for 800 msec depolarizing steps that elicited the maximum number of spikes under current clamp. Peak inward sodium current, steady-state outward potassium currents and currents evoked by ACh and choline were recorded under whole-cell voltage clamp. Values are provided.

附加文件13：源自UC-M、AP和UM诱导多能干细胞（induced pluripotent stem cell, iPSC）模型的体外培养神经元电生理特性比较（.xlsx格式）。数据分别展示了（A）所有细胞（皮质兴奋性神经元（cortical excitatory neurons, cExNs）和皮质抑制性神经元（cortical inhibitory neurons, cINs））、（B）皮质兴奋性神经元(cExNs)以及（C）皮质抑制性神经元(cINs)的相关结果。
对于（A）部分的各基因型细胞，数据列展示了整合各基因型的cExNs与cINs记录数据后，所有记录细胞各参数的中位数、第一四分位数、第三四分位数以及细胞计数（细胞数量）。右侧列则展示了神经元细胞类型特异性数据，即整合三种基因型的cExN数据或cIN数据得到的结果。浅红色色块标记代表通过双向秩和方差分析（two-way analysis of variance on ranks, 2-WAY ANOVA），以基因型（UC-M : AP : UM）与神经元细胞类型（cExN : cIN）为两个分析因素，采用Student-Newman-Keuls法进行事后多重比较，所得显著性差异结果（p < 0.05）。
对于（B）-（C）部分，（B）各基因型的皮质兴奋性神经元或（C）各基因型的皮质抑制性神经元的数值均已给出。棕褐色色块标记代表通过单向秩和方差分析（one-way analysis of variance on ranks, 1-WAY ANOVA），采用Student-Newman-Keuls法进行事后多重比较，所得显著性差异结果（p < 0.05）；其中深棕褐色表示某一种基因型与另外两种存在显著差异，浅棕褐色表示三种基因型中有两种彼此间存在显著差异。
膜电容与输入电阻通过-80 mV电位下的10 mV电压阶跃测得。锋电位阈值、幅值与时程半高宽均取自首次达到阈值时记录到的第一个动作电位。最大初始放电频率与最大平均放电频率通过可诱发最大动作电位数量的800 ms去极化电流阶跃（电流钳模式下）测得。峰值内向钠电流、稳态外向钾电流以及乙酰胆碱（acetylcholine, ACh）与胆碱诱发的电流均通过全细胞膜片钳电压钳模式记录。所有相关数值均已提供。