Construct design for the crystallisation of huntingtin fragments - 2018/06/11

Project - Huntingtin structure-function open lab notebook. Objective - Use the cryo-EM structure of huntingtin in complex with HAP40 http://www.rcsb.org/structure/6EZ8 to guide construct design of discrete domains and fragments which could be amenable to successful expression and purification of monodisperse protein samples and subsequent structure solution by X-ray crystallography.

项目：亨廷顿蛋白结构-功能开放实验笔记。研究目标：借助与HAP40结合形成复合物的亨廷顿蛋白（huntingtin）的冷冻电镜（cryo-EM）结构（收录于RCSB蛋白质数据库，结构编号6EZ8，详情见http://www.rcsb.org/structure/6EZ8），指导离散结构域与蛋白片段的构建设计，使所设计的结构域与片段可顺利实现单分散蛋白质样品的表达与纯化，并通过X射线晶体学完成后续的结构解析。