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Additional file 2 of Long-read sequencing and genome assembly of natural history collection samples and challenging specimens

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DataCite Commons2025-02-11 更新2025-05-07 收录
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Additional file 2: Table S1: Detailed list of species and samples, together with DNA extraction and long-read sequencing results of each SMRT cell. Table S2: Assembly statistics for Cathorops nuchalis and Cathorops wayuu. Table S3: High levels of contamination in samples of Russian desman, Hazel dormouse and slow worm. Table S4: Bradypus torquatus genome assemblies generated with data from ultra-low input libraries prepared with polymerases A/B and/or C at different coverages. Table S5: Genome assemblies generated with polymerases A/B and/or C data at different coverages for Elyisa timida, Scintilla philippinensis, and Podura aquatica. Table S6: Assembly statistics for all tested contig assemblers using sequenced PacBio HiFi reads from four SMRTs produced with polymerase A/B and one SMRT produced with polymerase C for Bradypus torquatus.

补充文件2: 表S1:物种与样本详细列表,以及每个SMRT细胞的DNA提取和长读长测序结果。 表S2:项鳍海鲶(Cathorops nuchalis)与瓦尤海鲶(Cathorops wayuu)的基因组组装统计数据。 表S3:俄罗斯麝鼹、榛睡鼠及慢缺肢蜥样本的高污染水平。 表S4:鬃三趾树懒(Bradypus torquatus)的基因组组装结果,其测序数据来自使用聚合酶A/B和/或C制备的超低起始量文库,且覆盖度各不相同。 表S5:针对伊氏钝绥螺(Elyisa timida)、菲律宾闪光螺(Scintilla philippinensis)与水跳虫(Podura aquatica),采用聚合酶A/B和/或C的测序数据、设置不同覆盖度所生成的基因组组装结果。 表S6:针对鬃三趾树懒(Bradypus torquatus),利用4个经聚合酶A/B制备的SMRT细胞与1个经聚合酶C制备的SMRT细胞产出的PacBio HiFi测序读段(PacBio HiFi reads),对所有测试过的重叠群组装工具进行组装后的统计数据。
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figshare
创建时间:
2025-02-11
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