The impact of Unc45A silencing on gene expression in the human breast cancer Hs578T (ATCC HTB-126) cell line

The co-chaperone Unc45A is essential for cancer cell growth. We show that Unc45A, a cytoplasmic protein in normal cells, becomes nuclear in cancer cells. We used Microarray to determine the impact of UNC45A on gene expression. We found that Unc45A unctions as a co-activator of the glucocorticoid receptor. In this capacity, it differentially promotes expression of the mitotic kinase NEK7 and other genes possibly through regulation of nuclear receptors. Human breast triple negative Hs578T cancer cells were transfected with siRNA against Unc45A or non-targeting siRNA control.