α1ACT is essential for survival and early cerebellar programming in a critical neonatal window [RNA-Seq]

Previous study has shown that alpha1ACT is a transcription factor involved with regulating neuronal gene expression. We performed a time-series RNA-seq study using pc12 cell lines stably expressing pcDNA3-alpha1ACT at 4 time points (6hr, 24hr, 3day, and 10day) to explore the transcriptional profiles that capture transient and prolonged dynamic changes regulated by alpha1ACT during cell cycle and differentiation既往研究表明，alpha1ACT是一种参与调控神经元基因表达的转录因子。本研究采用稳定表达pcDNA3-alpha1ACT的PC12细胞系，设置6小时、24小时、3天、10天共4个时间点开展时间序列RNA测序（RNA-seq）研究，以探究可捕捉alpha1ACT在细胞周期与细胞分化过程中所调控的瞬时及持续性动态变化的转录谱特征。