A Co(II)-based coordination polymer: treatment activity on endometrial cancer by inducing cell apoptosis through regulating the expression of miRNA29

A novel 3D Co(II) coordination polymer (CP) {Co3(IBT)2(H2O)2}n (1) has been synthesized through the reaction of 4,5-Bis(tetrazol-5-yl)imidazole (H3IBT) with Co(NO3)2 in a mixed solvent of N,N-dimethylformamide (DMF) and water. The structural determination results show that complex 1 is a 3D framework with a (3,4)-connected net. Furthermore, its inhibitory activity on endometrial cancer cells was evaluated and the related mechanism was also discussed in the research. The relative expression of the miRNA29 in endometrial cancer cells was measured with real-time PT-PCR. In addition to this, the western blot was performed to detect the endometrial cancer cell apoptosis after compound treatment for the mechanism exploration. The hypothesis that arises from the experimental observation has been further investigated by using molecular docking simulation, where the possible binding interactions between the ligand and Bcl2 protein are studied explicitly.