Host Factor Experiment (IM006B-R)

Purpose: To obtain lung samples from C57BL6 mice infected with Vietnam/1203-CIP048_RG3/2004 (H5N1) for both transcriptional and proteomic analyses. Details: Time Points = 1, 2, 4 and 7 days post infection; 5 replicates for infected mice and triplicate mice for the mocks; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^4 PFU.研究目的：从感染Vietnam/1203-CIP048_RG3/2004 (H5N1) 亚型禽流感病毒的C57BL/6小鼠中获取肺部样本，以开展转录组学与蛋白质组学分析。 实验细节：感染后采样时间点设为1、2、4和7天；感染组小鼠设置5个生物学重复，模拟感染组小鼠设置3个生物学重复；模拟感染所用的接种培养基与病毒感染所用培养基完全一致；病毒感染剂量为10^4 PFU（噬斑形成单位）。