Vast differences in organ-specific rhythms of transcription in field-grown sugarcane

Sugarcane (Saccharum hybrid, SP80-3280) was grown in the field in Araras (Brazil) for 9 months. Leaves +1 (F1), internodes 1&2 (I1), and internodes 5 (I5) were harvested every 2 h for 26 h, starting 2h before dawn. Samples were collected from 2h before dawn (00) of one day to 2 h after dawn of the next day (26). Leaves +1 (F1), internodes 1&2 (I1), and internodes 5 (I5) were harvested. Samples were taken every 2 h were labelled accordingly. Two biological replicates of each time point were made, with a dye swap with the reference. The reference used was an equimolar solution of all samples.