Profiling DNA G-quadruplex structures at single-cell level

Single-cell sequencing data uncovers cellular heterogeneity and enables studies of underlying molecular variations within cell population. Here, we adapted CUT&Tag to profile DNA secondary structure G-quadruplexes at single-cell resolution for the first time and single-cell G-quadruplex maps readily distinguished cell types in a mixed population and revealed variable frequency of G-quadruplexes observed in a given sample. Genome-wide distribution of DNA G-quadruplex structures were profiled with bulk or single nuclei G4 CUT&Tag protocol using BG4 antibody in three cell lines (K-562, U2OS, MCF-7). Technical and biological replicates have been performed to assess reproducibility and sensitivity.